Journal: Scientific Reports

Article Title: Anxiolytic effects of Enterococcus faecalis 2001 on a mouse model of colitis

doi: 10.1038/s41598-024-62309-3

Figure Lengend Snippet: Effects of EF-2001 on serum LPS and corticosterone, and p-GR in the brain. Effects of chronic treatment with Enterococcus faecalis 2001 (EF-2001) on increased serum LPS ( a ) and corticosterone ( b ) concentration, and enhanced p-GR in the prefrontal cortex (PFC) ( c ) and ventral hippocampus ( d ) of DSS-treated mice. Quantification of the normalized values of p-GR with t-GR. Original immunoblot images were shown in Supplementary Fig. . ( e ) Microscopy images of p-GR (Ser211) (green), DAPI (blue), and NeuN, GFAP or Iba1 (red) immunostaining in the PFC of DSS-treated mice. Bars represent means ± standard error of mean (SEM). *p < 0.05 and **p < 0.01 vs. water-treated water group, # p < 0.05 and ## p < 0.01 vs. water-treated DSS group (n = 6–8 per group).

Article Snippet: After washing three times every 5 min, the sections were incubated with PBS containing 1% normal goat serum (Life Technologies Corporation, Carlsbad, CA, USA) or 3% bovine serum albumin and 0.3% Triton X-100 (PBSGT or PBSBT) at room temperature (23 ± 1 °C) for 2 h. The sections were incubated overnight at 4 °C with rabbit anti-p-GR (Ser211) (1:200; Cell Signaling Technology, #4161), rabbit anti-p-CAMKII (1:500; Cell Signaling Technology, #3361), rabbit anti-p-CREB (1:200; Cell Signaling Technology, #9198), mouse anti-NeuN (1:200; Millipore, MAB377), mouse anti-glial fibrillary acidic protein (GFAP; 1:200; Millipore, MAB360), or goat anti-ionized calcium-binding adaptor molecule 1 (Iba1; 1:200, Abcam, ab5076).

Techniques: Concentration Assay, Western Blot, Microscopy, Immunostaining